2011年4月3日星期日

DNA extraction lab

On friday, the whole class did a Dna extraction lab, it was so much fun!  i'm just going to briefly talk about that lab as well as show some pictures which have been taken during the class.


so firstly, we are divided into to parts, one part of students do labs based on the strawberrys, the the other half do the labs on kiwi fruits. i and patrick were doing the strawberry one. basically, we put a few strawberrys into the hot water to warm it up in order to break the cell walls, the temperature should be no more than 55 degrees, otherwise it is gonna damage the cell. later on, after warming up, we take out the strawberrys and grind them into liquid, then add some buffer into it in order for the reaction to occur. then the most important part comes, by adding the alcohol to the mixed solution gently(naking sure the alcohol flow on the top of the solution, not mixing them), we finally extract the white substance on the top of the solution after waiting for a while, which is the DNA of the strawberry.
                                        the white substance on the top is the DNA

                                         lol~~~ 

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